I ran a 30-day stability test on reconstituted WWB semaglutide to answer the question everyone asks: "How long does reconstituted sema last in the fridge?" Methodology: - Product: WWB Semaglutide 10mg, batch #WS-SEM-2026-0203 - Reconstituted with 1mL bacteriostatic water (0.9% benzyl alcohol) - Stored at 4°C (standard refrigerator) in original vial - Sent aliquots to Janoshik at Day 0, Day 10, Day 20, and Day 30 - Each test was standard HPLC purity analysis 📋 Results: | Time Point | HPLC Purity | Degradation from Day 0 | |-----------|-------------|----------------------| | Day 0 | 99.1% | — | | Day 10 | 98.9% | -0.2% | | Day 20 | 98.5% | -0.6% | | Day 30 | 97.8% | -1.3% | Key Takeaway: WWB semaglutide maintained >97.5% purity through 30 days when reconstituted with BAC water and stored at 4°C. The degradation rate was approximately 0.043% per day, which is very reasonable. This means a reconstituted vial is perfectly usable for a full month. You could probably push it to 45 days before hitting 97% but I wouldn't go much beyond that. Total testing cost for this experiment: $540 (4x Janoshik panels). Expensive, but now we all have real data instead of guessing. 🔬

This is phenomenal data. $540 well spent for the community. A few questions about methodology: 1. Did you use a sterile technique when drawing aliquots? Cross-contamination could accelerate degradation. 2. Was the refrigerator temperature stable at 4°C or did it fluctuate (door opening, etc.)? 3. Did you notice any visual changes — cloudiness, particulates, color change? The 0.043%/day degradation rate is actually better than I expected. Some published literature on reconstituted semaglutide suggests 0.05-0.08%/day for generic formulations.

Great questions: 1. Yes, sterile technique throughout. Wiped stoppers with 70% IPA before each draw, used fresh sterile syringes, worked near a flame. I'm a biochem postdoc so this is second nature. 2. I used a dedicated mini-fridge with a digital thermometer logging temps every 30 min. Range was 3.2-5.1°C with an average of 4.1°C. No significant excursions. 3. No visual changes at any point. Solution remained perfectly clear and colorless from day 0 through day 30. > The 0.043%/day degradation rate is actually better than I expected I was pleasantly surprised too. The bacteriostatic water quality matters — I used Hospira brand BAC water, which is pharmaceutical grade. Cheap BAC water with impurities could accelerate degradation.

Let me translate this for the non-scientists: your reconstituted WWB sema is fine in the fridge for a month. Use bacteriostatic water, not plain sterile water. Keep it cold. You're good. Most people go through a 10mg vial in 2-4 weeks anyway at standard dosing, so this confirms what many of us assumed — the vial doesn't go bad before you finish it. Thanks for the breakdown, this is a genuinely useful contribution. 👏

I did a similar (less rigorous) test with QSC semaglutide last year and found roughly 0.06%/day degradation under similar conditions. So WWB's product appears to be slightly more stable post-reconstitution. This could be due to: - Higher initial purity (less impurities to catalyze degradation) - Better lyophilization process (affects reconstituted stability) - Differences in residual solvents/salts Whatever the reason, it's another data point in WWB's favor for quality. The lyophilization quality really does seem to matter for post-recon stability.