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ForumsCOA & Analytical TestingLC-MS/MS vs HPLC for peptide analysis — November 2025 Page 4

LC-MS/MS vs HPLC for peptide analysis — November 2025

TrialTracker_MD Wed, Jan 29, 2025 at 1:49 PM 39 replies 2,208 viewsPage 4 of 8
TrialTracker_MD
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Jan 29, 2025 at 7:29 PM#16

OP here — wow, did not expect this much response! Thank you all for the thoughtful replies on LC-MS/MS vs HPLC for.

To answer TrialNerd_Beth: yes, I am on compounded sema from a 503B pharmacy.

Quick update: things are still going well. Thanks again for the evidence-based discussion — this is exactly why I posted in a science-based community. 🙏

Last edited: Jan 30, 2025 at 12:29 AM
7 23Dr.EM_Chicago, pete_RVA, CarlaRPh_TPA and 4 others
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FDA_TrackerJim
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Jan 29, 2025 at 7:46 PM#17

Relevant to LC-MS/MS vs HPLC for peptide — here is my latest bloodwork comparison:

Key improvements: A1C 7.6% → 5.5%, triglycerides 205 → 105 mg/dL, hsCRP 8.0 → 1.1 mg/L. All on tirzepatide for 9 months.

The inflammatory marker drop is what impresses me most. Consistent with the SELECT trial's cardiovascular findings.

31 13Admin, Dr.Martinez, mike_mod and 28 others
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DadBodDave
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Nov 2024
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Jan 29, 2025 at 8:03 PM#18

100%. My provider said the same thing about LC-MS/MS vs HPLC for.

27 0pat_auckland, Dr.GastroMayo, JakeBK_lifts and 24 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

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HPLC_Greg
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Jan 29, 2025 at 8:20 PM#19

Slightly tangential to LC-MS/MS vs HPLC for but MaxMetOK reminded me — does anyone have experience combining this with metformin?

I know it is not exactly on-topic but it seems related enough to ask here rather than starting a new thread. Apologies if this has been covered — I did search first.

3 23Dr.MetabolicMD, RetaRick_CA, JenPlateau
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labquiet_amy
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Cambridge, MA
Jan 29, 2025 at 8:37 PM#20
TrialTracker_MD said:
Thank you all for the thoughtful replies on LC-MS/MS vs HPLC for

This is exactly right. TrialTracker_MD articulated what I have been trying to explain to my doctor for months. The LC-MS/MS vs HPLC for aspect is the most important factor.

14 7pete_manc_UK, anna.melb_AU, mark_tokyo and 11 others
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