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ForumsCOA & Analytical TestingLC-MS/MS vs HPLC for peptide analysis — November 2025 Page 9

LC-MS/MS vs HPLC for peptide analysis — November 2025

Dr.SportsMedIN Fri, Apr 26, 2024 at 3:37 PM 51 replies 2,260 viewsPage 9 of 11
KarenAZ_mom
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Scottsdale, AZ
Apr 27, 2024 at 4:22 AM#41

oliver_london — that is really helpful context on LC-MS/MS vs HPLC for. Follow-up question: did you experience any side effects during this phase?

I am in a similar situation (about to start) and trying to set realistic expectations.

42 17anna.melb_AU, mark_tokyo, hans_munich and 39 others
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laura_annarbor
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Dec 2024
Ann Arbor, MI
Apr 27, 2024 at 4:39 AM#42

Want to share my personal experience related to LC-MS/MS vs HPLC for peptide since I think it might help others in a similar situation.

I started my GLP-1 journey in March 2025 at 253 lbs with metabolic syndrome. My telehealth provider prescribed semaglutide 0.25mg to start.

Now, 16 months later: I am down 38 lbs, my labs have normalized, and my sleep apnea has improved dramatically. The LC-MS/MS vs HPLC for aspect specifically was something that made a big difference in my journey.

This forum was my go-to resource throughout.

Last edited: Apr 27, 2024 at 6:39 AM
23 21emily_PDX, Dr.SleepRoch, laura_annarbor and 20 others
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mia_MS2
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Mar 2026
Ann Arbor, MI
Apr 27, 2024 at 4:56 AM#43

KarenAZ_mom — that is really helpful context on LC-MS/MS vs HPLC for. Follow-up question: did you change anything about your diet at the same time?

I am in a similar situation (month 2) and trying to set realistic expectations.

38 2dave_SLC, FDA_TrackerJim, ricardo_MIA and 35 others
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bbq_ray_KC
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Jul 2024
Kansas City, KS
Apr 27, 2024 at 5:13 AM#44

Want to share my personal experience related to LC-MS/MS vs HPLC for peptide since I think it might help others in a similar situation.

I started my GLP-1 journey in October 2024 at 270 lbs with prediabetes and hypertension. My endocrinologist prescribed semaglutide 0.25mg to start.

Now, 8 months later: I am down 45 lbs, my labs have normalized, and I am off 2 of my 3 medications. The LC-MS/MS vs HPLC for aspect specifically was something that made a big difference in my journey.

This community helped me every step of the way.

12 14WendyG_ATL, SaraMom3, Dr.MetabolicMD and 9 others
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roxy_nash
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Dec 2024
Nashville, TN
Apr 27, 2024 at 5:30 AM#45

Just wanted to say — for anyone reading this LC-MS/MS vs HPLC for thread who is feeling discouraged: it gets better. The first 6 weeks are the hardest. Hang in there. 💪

Last edited: Apr 27, 2024 at 11:30 AM
44 6kim_atl_prep, sarah_TO, wendy_avl and 41 others
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